Thymidine-CPG-Support 500 Å pore size CPG/Supports are carriers for automated DNA synthesis. Mostly derivatized Controlled Pore Glass (CPG) is used. The 500 Å supports are suitable for almost all applications. In order to avoid steric obstacles between the growing oligonucleotide chains and thus obtain the highest possible yield of full-length chains, a long-chain alkylamine "spacer arm" is used.
Adenosine-CPG-Support 500 Å pore size CPG/Supports are carriers for automated DNA synthesis. Mostly derivatized Controlled Pore Glass (CPG) is used. The 500 Å supports are suitable for almost all applications. In order to avoid steric obstacles between the growing oligonucleotide chains and thus obtain the highest possible yield of full-length chains, a long-chain alkylamine "spacer arm" is used.
Oxidation reagent for DNA synthesis 0,05 M Iodine The phosphite triester linkage formed during oligonucleotide synthesis is of limited stability and has to stabilised. Treatment of the support-bound material with iodine and water in the presence of a weak base oxidizes the phosphite triester into a tetracoordinated phosphate triester, a protected precursor of the naturally occurring phosphate diester internucleosidic linkage.
Guanosine-CPG-Support 500 Å pore size CPG/Supports are carriers for automated DNA synthesis. Mostly derivatized Controlled Pore Glass (CPG) is used. The 500 Å supports are suitable for almost all applications. In order to avoid steric obstacles between the growing oligonucleotide chains and thus obtain the highest possible yield of full-length chains, a long-chain alkylamine "spacer arm" is used.
DMT Removal DCE for DNA synthesis Dimethoxytrityl is a protective group for hydroxy groups which is used to protect the 5' OH group of the individual nucleotides in DNA synthesis. The protective group is acid labile and is removed in the ongoing synthesis reaction by the addition of trifluoroacetic acid (deprotection) to enable the coupling of the subsequent nucleotide. We offer DMT-Removal-Reagent in dichloroethane (K060) as well as in dichloromethane (2257). DMT in DCE is used for synthesizers which draw the solution from the bottles by vacuum pumps and push it through the reaction columns. Since these vacuum pumps apply a significant force, the DCE is more suitable due to its high boiling pressure, as it is absorbed and introduced into the reaction without the formation of gas bubbles. The use of DMT in DCM could interfere with the reaction by creating gas bubbles.
Oxidation reagent for DNA synthesis 0,05 M Iodine The phosphite triester linkage formed during oligonucleotide synthesis is of limited stability and has to stabilised. Treatment of the support-bound material with iodine and water in the presence of a weak base oxidizes the phosphite triester into a tetracoordinated phosphate triester, a protected precursor of the naturally occurring phosphate diester internucleosidic linkage.