Substrates for Western and ELISA

604-44-4, Carl Roth

4-Chloro-1-naphthol ≥97 %, for biochemistry

604-44-4, Carl Roth

4-Chloro-1-naphthol ≥97 %, for biochemistry

207738-08-7, Carl Roth

3,3',5,5'-Tetramethylbenzidine dihydrochloride ≥99 %, for biochemistry Tetramethylbenzidine dihydrochloride is used as highly sensitive substrate for detection of horseradish peroxidase labelled probes, particularly in solution-based assays like ELISA techniques. It produces a soluble, blue-coloured end product, which can be read spectrophotometrically. The dihydrochloride form is well water-soluble , making it a versatile tool in laborartory science. Directions for use Working solution: Immediately prior to use add 2 µl / 10 ml of 30 % hydrogen peroxide (Liem et al. (1979) Anal Biochem , 98:388-93).

124-09-4, Carl Roth

124-09-4, Carl Roth

124-09-4, Carl Roth

39YC.1, Carl Roth

ROTI ® LumiX One, Basic for protein chemistry, ready-to-use ROTI ® LumiX One is a product line of single-component chemiluminescence substrates for Western blotting. The ROTI ® LumiX One products are available in various sensitivity levels to meet all detection requirements, ranging from picograms to low femtograms. Our pre-mixed solution, based on proprietary technology, ensures enhanced experimental consistency while eliminating pipetting errors and potential contamination. Applications: Compatible with all chemiluminescence detection devices and X-ray film detectors. Well-suited for various membranes, blocking reagents, and a wide range of antibody dilutions. Optimized for low background and high signal-to-noise ratio. Not a medical device / Not an IVD product

39YA.1, Carl Roth

ROTI ® LumiX One, Advanced for protein chemistry, ready-to-use ROTI ® LumiX One is a product line of single-component chemiluminescence substrates for Western blotting. The ROTI ® LumiX One products are available in various sensitivity levels to meet all detection requirements, ranging from picograms to low femtograms. Our pre-mixed solution, based on proprietary technology, ensures enhanced experimental consistency while eliminating pipetting errors and potential contamination. Applications: Compatible with all chemiluminescence detection devices and X-ray film detectors. Well-suited for various membranes, blocking reagents, and a wide range of antibody dilutions. Optimized for low background and high signal-to-noise ratio. Not a medical device / Not an IVD product

54827-17-7, Carl Roth

3,3',5,5'-Tetramethylbenzidine ≥98 %, p.a. Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine. Dissolves easily in acetone, chloroform and ethanol. Directions for use Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H 2 O 2 ad 0.01 %. Always prepare freshly! Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H 2 SO 4 and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187). Protect from light.

1128.3, Carl Roth

Exactly pre-weighed tablets for convenient and rapid preparation of pNPP solution. Prepared solution may be sterilized by filtration (0,22 µm). The solution has to be stored at -20 °C in aliquots. ROTI ® Fair Products - Sustainability As ready-to-use mixtures, ROTI ® Fair products reduce the user’s exposure to health-damaging substances used for preparation or pH adjustment. The production of ROTI ® Fair tablets and sachets is carefully controlled in large quantities and can be made energy efficient. ROTI ® Fair products reduce the total amount of waste produced and improve the footprint in the climate balance. ROTI ® Fair pNPP 20mg 20 mg/tablet, for biochemistry

P078.1, Carl Roth

Sensitive and reliable For all standard membrane types Low background No cumbersome preparation of peroxidase buffers Suitable for multiple stripping and hybridisation ROTI ® Lumin is a kit for chemoluminescent detection of peroxidase-linked reporters (e.g. HRP-antibodies), as can be found in Western-Blot assays. In comparison to chromogen substrates, ROTI ® Lumin displays increased sensitivity and reduced background reaction. Mechanism ROTI ® Lumin is transformed by the HRP into an excited dianion in the presence of hydrogen peroxide. When the dianion is reversed back to its normal state, light is emitted. The light emission of ROTI ® Lumin reaches its maximum within 5 minutes and remains at the maximum level for approx. 1-2 hours. ROTI ® Lumin 10x conc., fluorescence-free, for protein and cytoimmunochemistry Directions for use ROTI ® Lumin consists of two solutions, which should be mixed at a 1:1 ratio before use. It can be used with nitrocellulose, nylon and PVDF membranes. Optimal results can be achieved with Immobilon-P ® PVDF or ROTI ® PVDF. Can be used for Western-Blotting, ELISA, Southern- Blotting, Dot-Blotting and colony hybridisation. Membranes may be stripped from bound probes using ROTI ® Free stripping buffer, and may thus undergo multiple reprobing. Figure: Sensitivity of the ROTI ® Lumine. Sensitivity of ROTI ® Lumin ultra is found in low femtogram range. Detection of a polyclonal antibody (left to right 4 pg, 400 fg, 40 fg, 4 fg) on ROTI ® PVDF (Art. No. T830.1). Staining in PBST via a HRP-conjugated anti-rabbit antibody (1:2000). a/A: ROTI ® Lumin ultra; b/B: ROTI ® Lumin plus; c/C: ROTI ® Lumin; d/D: Product of a competitor, recommended for femtogram range. a/b/c/d: 10 sec. exposition, A/B/C/D: 1 min. exposition. The kit contains 120 ml (25 ml) ROTI ® Lumin 1 (Art. No. P079) and 120 ml (25 ml) ROTI ® Lumin 2 (Art. No. P080). Contents of this Kit may not be bought separately.

54827-17-7, Carl Roth

3,3',5,5'-Tetramethylbenzidine ≥98 %, p.a. Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine. Dissolves easily in acetone, chloroform and ethanol. Directions for use Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H 2 O 2 ad 0.01 %. Always prepare freshly! Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H 2 SO 4 and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187). Protect from light.