Distinguished by its well-documented behavior in regulated processes, the Roti ® Lux Onestep RT-qPCR Master Mix is designed for probe-based real-time PCR assays. This versatile solution includes all necessary components for cDNA synthesis, allowing direct use of isolated RNA as starting material. Featuring a HotStart DNA Polymerase, this master mix ensures efficient amplification while maintaining a low ROX version for optimal results. With a density of 1 g/cm³, it is easily handled and stored at -20 °C. Additionally, the amplicon ends are finished with 3'dA, ensuring precise results in your qPCR experiments.

• Category: qPCR
• Melting point (mp): 0 °C
• Boiling point (bp): 100 °C
• Polymerase: HotStart DNA Polymerase
• Instructions for use, addition: The master mix contains all components for reverse transcription and qPCR. Only primer and template must be added separately. 1 ml Master Mix is sufficient for one 96-well plate.
• Amplicon ends: 3'dA
• Storage temp.: -20 °C
• Version: low ROX
• Basic material: RNA
• Application: Probe-based real-time PCR assays
• Density (D): 1 g/cm³