Hepes belongs to a class of compounds with well-established chemical utility, characterized by the molecular formula C8H18N2O4S and a molecular weight of 238.31. This compound, known as 4-(2-hydroxyethyl)piperazin-1-ylethanesulfonic acid, falls under various substance groups such as aminosulphonic acids, hydroxy compounds, alcohols, and nitrogen heterocycles. Its specific role within these groups is confirmed through extensive research, making it an essential component in numerous applications. In professional settings, Hepes requires careful handling due to its chemical nature. It finds significant use in reagents for protein isolation, particularly in the reduction of clotting time during analysis of factors V and VIII, during APTT and PT tests, and during thrombin mediated clotting in plasma. Additionally, it serves as a reagent in plasma urea determination and a buffer reagent with high buffer capacity at physiological pH-values.

• CAS Number: 7365-45-9
• Molecular Formula: C8H18N2O4S
• Molecular Weight: 238.31
• Exact Mass: 238.09872823