3,3',5,5'-Tetramethylbenzidine Dihydrochloride is a structurally characterized compound with a molecular formula of C16H22Cl2N2 and a molecular weight of 313.3. This compound belongs to the class of aromatic amines, specifically tetramethylbenzidines. It serves as a highly sensitive substrate for the detection of horseradish peroxidase labelled probes, particularly in solution-based assays such as ELISA techniques. Upon reaction, it generates a soluble, blue-coloured end product that can be measured spectrophotometrically. As with many chemical substances, it carries a Warning signal word due to its potential acute toxicity when ingested.

• CAS Number: 207738-08-7
• Molecular Formula: C16H22Cl2N2
• GHS Signal Word: Warning
• Molecular Weight: 313.3
• Exact Mass: 312.1160041
• Hazard Statements:
• H302: Harmful if swallowed [Warning Acute toxicity, oral]