The bands of the marker are prestained with the fluorescent dye SYBR ® Green I, which has been developed specifically for DNA analysis. The dye bounds to the minor groove of DNA and also intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, the nucleic acid-bound dye emits a brightly coloured green fluorescence that may be documented by all usual broadband ethidium bromide filters or SYBR ® Green foto filters. 100 bp Fluorescent DNA Ladder 52.2 µg/500 µl, ready-to-use, prestained with Orange G and Xylene cyanol FF dye The 100 bp fluorescent DNA ladder is a ready-to-use DNA ladder that is premixed with a highly sensitive DNA-binding fluorescent dye and a loading dye for direct gel loading. Not a medical device / Not an IVD product
pUC19/Msp I lyophilised, not prestained Irregular DNA-marker for analysis of small DNA fragments. The marker was manufactured using pUC19-DNA and restriction endonuclease Msp I. Made from natural plasmids: fragment sizes can be validated! The concentration of the individual fragments is equimolar so that rough quantitation of the analysed DNA is possible. Fig.: 1 % agarose (NEEO, Art. No. 2267) + 1 % Synergel ™ (Art. No. 0184).
1 kbp DNA-Ladder lyophilised, not prestained Universal ladder for electrophoretic separation of fragments between 500 bp and 10 kb. Optimally suited for size determination of inserts, plasmids, and restricted DNA. The mass of the larger fragments was reduced, allowing to obtain clear and distinct bands with short running distances even for the large marker fragments. The terminal sticky Eco R I-restriction sites allow simple labelling of the marker fragments via end-labelling techniques. Directions for use The 1 kbp DNA-ladder is highly compatible with the 100 bp DNA-ladder (equalised: T833.1). Fig.: 1.2 % agarose (NEEO, Art. No. 2267). Additional band at 125 bp.
The bands of the marker are prestained with the fluorescent dye SYBR ® Green I, which has been developed specifically for DNA analysis. The dye bounds to the minor groove of DNA and also intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, the nucleic acid-bound dye emits a brightly coloured green fluorescence that may be documented by all usual broadband ethidium bromide filters or SYBR ® Green foto filters. 1 kb Fluorescent DNA Ladder 50 µg/500 µl, ready-to-use, prestained with Bromophenol blue and Xylene cyanol FF dye The 1 kb fluorescent DNA ladder is a ready-to-use DNA ladder that is premixed with a highly sensitive DNA-binding fluorescent dye and a loading dye for direct gel loading. Not a medical device / Not an IVD product
20 bp-DNA-Ladder ready-to-use, not prestained Regular DNA ladder for the analysis of very small DNA fragments (left lane in figure). The 100 bp fragment has been enhanced for easy analysis of the gel. All fragments are quantified, enabling quantitation of sample DNA in the gel. In order to avoid overlay of bands by bromophenol blue or xylene cyanol, Orange G was included as dye. Directions for use We recommend use of this ladder on the following special agaroses: High Resolution (Art. No. K297), HR-PLUS (Art. No. HP30), LM/PCR (Art. No. HP31), or an agarose with additional Synergel ™ (Art. No. 0184). The 20 bp DNA-Ladder is highly compatible with the 100 bp DNA-Ladder equimolar (Art. No. T834). By combining both, a versatile ladder from 20 bp up to 1000 bp is obtained (right lane in figure). Fig.: 1,4 % agarose (High Resolution, Art. No. K297) + 0,8 % Synergel ™ (Art. No. 0184) in 1 x TAE.
The bands of the marker are prestained with the fluorescent dye SYBR ® Green I, which has been developed specifically for DNA analysis. The dye bounds to the minor groove of DNA and also intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, the nucleic acid-bound dye emits a brightly coloured green fluorescence that may be documented by all usual broadband ethidium bromide filters or SYBR ® Green foto filters. 25 kb Fluorescent DNA Ladder 51.6 µg/500 ml, ready-to-use, prestained with Bromophenol blue and Xylene cyanol FF dye The 25 kb fluorescent DNA ladder is a ready-to-use DNA ladder that is premixed with a highly sensitive DNA-binding fluorescent dye and a loading dye for direct gel loading. Not a medical device / Not an IVD product
100 bp-DNA-Ladder equalized lyophilised, not prestained Regular DNA Ladder for standard small fragment applications. Ideal for analysis of fragments ≤500 bp. The DNA-quantity per band was set in such a way that the intensity of the individual bands is comparable (equalized). Fig.: 1,8 % agarose (NEEO, Art. No. 2267)
100 bp-DNA-Ladder equimolar lyophilised, not prestained Regular DNA Ladder for standard small fragment applications. The individual bands of the ladder are present in the mixture in the same molar concentration (equimolar). Fig.: 1,8 % agarose (NEEO, Art. No. 2267)
DNA-Marker short-run 1 lyophilised, not prestained The perfect DNA-marker for very short gel running distances and high-through-put approaches. The fragment mass is optimised for very short runs. The 1500 bp fragment has been enhanced for easy analysis of the gel. Directions for use Best results are already obtained at 18 mm running distance in a 1,5 % agarose gel (see figure). Fig.: 1,5 % agarose (NEEO, Art. No. 2267). Left - running distance 18 mm, Right - running distance 40 mm.
100 bp-DNA-Ladder equalized lyophilised, not prestained Regular DNA Ladder for standard small fragment applications. Ideal for analysis of fragments ≤500 bp. The DNA-quantity per band was set in such a way that the intensity of the individual bands is comparable (equalized). Fig.: 1,8 % agarose (NEEO, Art. No. 2267)
pBR328-Marker ready-to-use ready-to-use, not prestained Irregular DNA-marker for analysis of small DNA fragments. The marker was manufactured by mixing fragments obtained by restriction digest of pBR328 using the endonucleases Hinf I and Bgl I. Directions for use Made from natural plasmids: fragment sizes can be validated! The concentration of the individual fragments is equimolar so that rough quantitation of the analysed DNA is possible. Fig.: 1,5 % agarose (NEEO, Art. No. 2267).
pUC19-Marker ready-to-use ready-to-use, not prestained Irregular DNA-marker for analysis of small DNA fragments. The marker was manufactured using pUC19-DNA and restriction endonuclease Msp I. Made from natural plasmids: fragment sizes can be validated! The concentration of the individual fragments is equimolar so that rough quantitation of the analysed DNA is possible. Fig.: 1 % agarose (NEEO, Art. No. 2267) + 2 % Synergel ™ (Art. No. 0184)