Water soluble Mounting Media

4582.4, Carl

4274.3, Carl

0718.2, Carl

DABCO ® ≥99 % DABCO is an anti-bleaching reagent. It is combined with hydrophilic mounting media as Mowiol 4-88, Art. No. 0713, or glycerol in order to conserve the colours and the fluorescence of durable samples. Directions for use Solubility may change depending on the batch. If required stir solution constantly overnight at a temperature of 40-60 °C.

6471.1, Carl

Phytohistol ® plus ready-to-use, for microscopy Phytohistol ® plus is an aqueous mounting medium based on sugar with the consistency of liquid honey. It is especially suitable for plant materials. After evaporation of the water a rubber-like film remains. New compound High stability No hazardous ingredients Neutral pH 6-7 Directions for use Drop on slide, add specimen. When free from air bubbles place cover slip. Notes on storage For producing durable specimens we recommend to seal the edges of the cover slip with our ROTI ® Seal cover slip sealant (Art. No. 6473). Shelf life: The solution contains a preserving agent and is stable for minimum 4 years after manufacture.

4274.1, Carl

4582.1, Carl

2848.2, Carl

ROTI ® Mount Aqua ready-to-use, for microscopy ROTI ® Mount Aqua is an aqueous solution of polyacrylate resin used for mounting of paraffin sections and cryostat sections directly after staining. The solution is ready to use. Colours and tissue structures are preserved in an optimal way. For long-term preserving of fluorescent dyes we recommend ROTI ® Mount FluorCare (Art. No. HP19). Directions for use Residues of staining solution are removed by rinsing with buffer or destilled water. Afterwards the specimens are mounted with ROTI ® Mount Aqua. Notes on storage Store at room temperataure, protected from light.

P049.1, Carl

HP19.1, Carl

ROTI ® Mount FluorCare ready-to-use, for fluorescence microscopy ROTI ® Mount FluorCare has been developed especially for fluorescene microscopy and is compatible with all current fluorescent dyes. It is an aqueous mountant with an additional antifading reagent which reduces photobleaching drastically. Fluorescence will remain for many weeks. In contrast to other mounting media ROTI ® Mount FluorCare does not show autofluorescence at any common wavelength. ROTI ® Mount FluorCare is also available with the dyes DAPI (HP20.1) and propidiumiodide (HP21.1) for simultaneous counter staining of nuclei. Filled in dropper bottles. Refractive index (20 °C): 1.44-1.465 Prevents photobleaching Compatible with a wide range of fluorescent dyes Example of Use Figure: Immunostaining of DNA adducts (red) and CNPase (green) in cryosections of the inner ear ( stria vascularis ) from mice a) mounted in glycerol b) mounted in ROTI ® Mount FluorCare With kind assistance of laboratory Dr. J. Thomale, DNA-Repair Group, Institute of Cell Biology, West German Cancer Centre Essen. Notes on storage The solution should be stored at +4 °C, protected from light.

6474.1, Carl

Kaiser’s glycerol gelatine phenol-free for microscopy It is an aqueous mounting medium which is solid at room temperature. Composition and application are according to the classical Kaiser’s glycerol gelatine; however, it does not contain phenol. The added preserving agent is classified as non-hazardous. The pH value of the product is 6-7. Therefore, it is suitable also for stainings which are sensitive against acids (e.g. hematoxylin). Non-hazardous ingredients Neutral pH (6-7) Convenient also for sensitive stainings Directions for use Prior to use the glycerol gelatine is melted on a water bath (max. 50-55 °C) and then dropped onto the slide by using a pipette or a glass rod.

0713.2, Carl

Mowiol ® 4-88 granulated For durable preparations. Mowiol 4-88 is used as hydrophilic mounting medium, particularly for immune histochemical applications. Usually it is applied in combination with the antibleaching reagent DABCO (1.4-Diazabicyclo-(2.2.2)octane), Art. No. 0718, which conserves the colours and the fluorescence of durable samples.

0718.9, Carl

DABCO ® ≥99 % DABCO is an anti-bleaching reagent. It is combined with hydrophilic mounting media as Mowiol 4-88, Art. No. 0713, or glycerol in order to conserve the colours and the fluorescence of durable samples. Directions for use Solubility may change depending on the batch. If required stir solution constantly overnight at a temperature of 40-60 °C.