Reagents for Decalcification

3738.4, Carl

4625.1, Carl

3744.3, Carl

Trichloroacetic acid ≥99 %, Ph. Eur., extra pure

6483.3, Carl

Tissues containing solid material such as bones or keratin are to be decalcified before paraffin embedding. Usually the specimens are treated with acids of different concentrations and mixtures. An especially gentle method is decalcification with EDTA solution. Decalcifier standard ready-to-use, for histology The solution contains trichloroacetic acid and formalin and, therefore, has also fixing qualities. Nevertheless, we recommend sufficient fixing of specimens before decalcifying for trichloroacetic acid may cause morphological changes in unfixed tissue. The length of the decalcification process depends on the specimen’s size and type. Little bones (diameter a few mm) need 1-2 days for complete decalcification. The decalcifier standard is not suitable for immunological applications. We recommend our Decalcifier soft (Art. No. 6484) on base of EDTA. Not a medical device / Not an IVD product

6484.2, Carl

Tissues containing solid material such as bones or keratin are to be decalcified before paraffin embedding. Usually the specimens are treated with acids of different concentrations and mixtures. An especially gentle method is decalcification with EDTA solution. All SOLVAGREEN ® products as well as further information about the product can also be found in our current flyer Green Chemistry - Alternative Solvents and Products . Decalcifier soft SOLVAGREEN ® ready-to-use, for histology The solution contains 25 % EDTA. The process of decalcification takes more time than acid decalcification (see Decalcifier standard, Art. No. 6483), e.g. bone biopsies need approx. 3-4 days. The procedure can be accelerated by using a magnetic stirrer. The decalcification takes place in a weakly basic milieu. Nucleic acids and enzymes are not affected; therefore, the solution is also suitable for immunological applications. Sustainability Decalcifier solution on base of EDTA Not a medical device / Not an IVD product

4625.3, Carl

6483.2, Carl

Tissues containing solid material such as bones or keratin are to be decalcified before paraffin embedding. Usually the specimens are treated with acids of different concentrations and mixtures. An especially gentle method is decalcification with EDTA solution. Decalcifier standard ready-to-use, for histology The solution contains trichloroacetic acid and formalin and, therefore, has also fixing qualities. Nevertheless, we recommend sufficient fixing of specimens before decalcifying for trichloroacetic acid may cause morphological changes in unfixed tissue. The length of the decalcification process depends on the specimen’s size and type. Little bones (diameter a few mm) need 1-2 days for complete decalcification. The decalcifier standard is not suitable for immunological applications. We recommend our Decalcifier soft (Art. No. 6484) on base of EDTA. Not a medical device / Not an IVD product

4724.4, Carl

4989.4, Carl

3744.2, Carl

Trichloroacetic acid ≥99 %, Ph. Eur., extra pure

6483.1, Carl

Tissues containing solid material such as bones or keratin are to be decalcified before paraffin embedding. Usually the specimens are treated with acids of different concentrations and mixtures. An especially gentle method is decalcification with EDTA solution. Decalcifier standard ready-to-use, for histology The solution contains trichloroacetic acid and formalin and, therefore, has also fixing qualities. Nevertheless, we recommend sufficient fixing of specimens before decalcifying for trichloroacetic acid may cause morphological changes in unfixed tissue. The length of the decalcification process depends on the specimen’s size and type. Little bones (diameter a few mm) need 1-2 days for complete decalcification. The decalcifier standard is not suitable for immunological applications. We recommend our Decalcifier soft (Art. No. 6484) on base of EDTA. Not a medical device / Not an IVD product

3738.2, Carl