Antibiotics

0240.2, Carl

Puromycin dihydrochloride ≥98 %, for biochemistry Inhibits protein biosynthesis by premature release of the nascent polypeptide chain. Functions as analogon to amino acyl-tRNA molecules.

8682.1, Carl

Cycloheximide ≥90 %, for biochemistry Antibiotic isolated from Streptomyces griseus . Its main biological activity is translation inhibition in eukaryotes by binding to the 60S subunit of the ribosomes. Cycloheximide inhibits binding of Aminoacyl-tRNA to the ribosomes, Peptidyltransferase, detachment of deacylated tRNA, and translocation of Aminoacyl-tRNA, resulting in cell growth arrest and apoptotic cell death. Not effective against bacteria. Cycloheximide is widely used for selection of cycloheximide-resistant strains of yeast and fungi, for inhibition of eukaryotic contaminants in bacterial microbiology, for controlled inhibition of protein synthesis in cell culture and for detection of short-lived proteins, and for apoptosis induction or facilitation of apoptosis induction by death receptors.

0237.2, Carl

Tetracycline hydrochloride ≥900 μg/mg, for biochemistry and molecular biology Effectual against gram-positive and -negative bacteria. Inhibits bacterial protein biosynthesis by coupling to amino acyl-tRNA. Modern application: Activation or inhibition of gene expression through tetracyclin-regulated promotor systems in vitro and in vivo . Light-sensitive.

23PT.2, Carl

8969.1, Carl

Actinomycin D ≥950 μg/mg, for biochemistry Specific inhibitor of DNA-dependent RNA-synthesis. Inhibition is caused by intercalation in dsDNA and, therefore, is not specific for procaryotic cells. Standard application is the preparation of cDNA. Actinomycin D is cytostatic, toxic and sensitive to light. Stock solution should be kept in a bottle covered with foil.

0242.7, Carl

Vancomycin hydrochloride CELLPURE ® ≥900 U/mg Inhibits bacterial cell cleavage through binding to cell membrane pre-stages. Endotoxins: ≤0.33 E.U./mg

6344.2, Carl

Carbenicillin disodium salt ≥88 %, for biochemistry Carbenicillin inhibits cell wall biosynthesis in bacteria. Compared to ampicilline, carbenicillin is more resistant to hydrolysis by β-lactamase.

0236.1, Carl

Streptomycin sulphate >720 I.U./mg, for biochemistry Effectual against mycobacteria, many gram-negative cocci and gram-negative bacteria in general. Results in translation errors by interaction with ribosomal 30S subunits.

CN28.2, Carl

Penicillin G sodium salt ≥1500 I.U./mg, for biochemistry Inhibits cell wall biosynthesis in gram-positive bacteria by irreversible interaction with transpeptidase. Application equivalent to Penicillin G potassium salt. Preferable when addition of potassium is to be avoided.

3011.2, Carl

Nourseothricin ≥85 %, for biochemistry and microbiology Antibiotic of the streptothricine family, effective against a broad spectrum of viruses, bacteria and fungi. Used as selection antibiotic for genetically modified bacteria, yeasts, filamentous fungi, protozoa and plants. Nourseothricin inhibits biosynthesis and induces miscoding. Resistance is mediated by the sat1 encoded N-acetyltransferase, which inactivates nourseothricin by acetylation of the β-amino group of the β-lysin. Streptothricine F: n=1 (MW 599.59), Streptothricine D: n=3 (MW 857.95) Recommendation: One package Nourseothricin should be solubilized in whole and the stock solution should be frozen in aliquots. By removing the overlaying argon during weighing, the stability of the lyophilsate is reduced.

T832.5, Carl

Kanamycin ≥750 I.U./mg, for biochemistry Kanamycin inhibits protein biosynthesis and is effective against gram-negative and gram-positive bacteria. Easily water-soluble, stable powder.

28LA.2, Carl

Cefotaxime sodium salt 916 - 964 μg/mg dried basis , USP Cefotaxime is a beta-lactamase-resistant antibiotic from the cephalosporin class. The antibiotic acts by binding to penicillin-binding proteins and thus inhibits bacterial cell wall synthesis. Not a medical device / Not an IVD product