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Sterile Prepacked ROTI ® Dex-Spin columns for size exclusion chromatography by centrifugation, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions. For preparation of small sample volumes (optimal 50 µl) in less than 5 minutes. The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin. In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules. In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins. Advantages: Sample preparation in just a few minutes Desalting, removal of impurities and buffer exchange in a single step No interaction between analyte and medium High selectivity, high resolution and high yield (> 95%) Also suitable for small sample volumes High chemical stability and therefore compatible with many organic and other solvents Size Exclusion Chromatography Columns ROTI ® Dex-25 Medium Spin The column bed consists of ROTI ® Dex-25 Medium (Art. No. 21A5), pre-swollen in deionised water. Suitable for: Oligonucleotides > 10 bp Globular proteins > 5 kDa Particles > 2 nm For optimal results, a sample volume of 50 µl is recommended. Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
Product Detail
Sterile Prepacked ROTI ® Dex-Spin columns for size exclusion chromatography by centrifugation, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions. For preparation of small sample volumes (optimal 50 µl) in less than 5 minutes. The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin. In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules. In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins. Advantages: Sample preparation in just a few minutes Desalting, removal of impurities and buffer exchange in a single step No interaction between analyte and medium High selectivity, high resolution and high yield (> 95%) Also suitable for small sample volumes High chemical stability and therefore compatible with many organic and other solvents Size Exclusion Chromatography Columns ROTI ® Dex-50 Medium Spin The column bed consists of ROTI ® Dex-50 Medium (Art. No. 21A6), pre-swollen in deionised water. Suitable for: Oligonucleotides > 20 bp Globular proteins > 25 kDa Particles > 4 nm For optimal results, a sample volume of 50 µl is recommended. Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
