Amido black 10 B (C.I. 20470), 25 g

Developed to align with modern chemical standards, Amido Black 10 B (C.I. 20470) serves as an essential blotting reagent in life science applications. This compound, characterized by its Molecular Formula C22H14N6Na2O9S2 and Molecular Weight of 616.50 g/mol, is compatible with PVDF and NC membranes. Its redox potential E of +0.57 volts indicates its ability to participate in oxidation-reduction reactions. The recommended staining solution for this reagent is 0.5% in 50% methanol and 10% acetic acid, with a suggested staining time of 1-5 minutes at room temperature. Background reduction can be achieved by soaking the stained material in 50% methanol at room temperature.

• Category: Blotting Reagents
• Ox. colour: yellow-brown
• CAS No.: 1064-48-8
• Empirical formula: C22H14N6Na2O9S2
• Density (D): 1,536 g/cm³
• Redox potential E: +0.57
• Molar mass (M): 616,50 g/mol
• Red. colour: blue