Known for its stable chemical composition in standard conditions, the Ripa Buffer is a versatile solution for the lysis of mammalian cells. This buffer efficiently extracts and solubilizes proteins from various cellular compartments such as the membrane, the cell nucleus, and the cytoplasm. A single milliliter of this buffer can effectively lyse between 0.5 and 5 million cells, making it suitable for both adhesion and suspension cultures. For optimal results, Additionally, protease and phosphatase inhibitors can be added to the lysis buffer as necessary to preserve protein integrity during the lysis process.

• Category: Cell lysis
• Density (D): 1,052 g/cm³
• Application: 1 ml RIPA buffer is sufficient for the lysis of 0,5 to 5 x 10 7 cells (75 cm 2 cell layer).
• Storage temp.: +2 to +8 °C
• Instructions for use, addition: Protease and phosphatase inhibitors may be added to the lysis buffer as needed.