Built to deliver predictable results in synthesis routines, the Roti ® Prep Plasmid Mini-XL offers a compact solution for isolating plasmids from 0.5 to 15 milliliters of bacterial cultures. This centrifugation-based extraction kit, available in ten preparations, employs an alkaline lysis method and utilizes a spin filter for efficient plasmid binding. The typical yield ranges from less than 20 micrograms for 2 milliliters to less than 80 micrograms for 15 milliliters. The elution volume is between 50 and 100 microliters, and the preparation time is approximately 20 minutes. The kit stores optimally at temperatures between 15 and 25 degrees Celsius. The typical purity, as measured by A260/A280 ratio, falls within the range of 1.7 to 2.0.

• Category: Centrifugation-based extraction kits
• Typical purity (A260/A280): 1.7-2.0
• Typical yield: <20 μg for 2 ml, <80 μg for 15 ml
• Basic material: Bacteria and yeast
• Preparation time: approx. 20 min
• Version: Mini prep
• Storage temp.: +15 to +25 °C
• Quantity of material: 0.5 - 15 ml
• Automatic use: no
• Density (D): 1,05 g/cm³
• Elution volume: 50-100 µl