4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(β-D-galactopyranosyl)-β-D-glucopyranoside, 1 mg

4-Nitrophenyl 2-Acetamido-2-Deoxy-4-O-(Β-D-Galactopyranosyl)-Β-D-Glucopyranoside, available in a 1 mg pack, is a chromogenic substrate with the molecular formula C19H21NO11 and molecular weight of 435.34 g/mol. This compound belongs to the class of glycosides, specifically those containing both glucose and galactose residues. It serves as a substrate in enzymatic assays, particularly those involving β-galactosidases, due to its ability to undergo hydrolysis upon enzymatic cleavage. Upon hydrolysis, it produces 4-nitrophenol, which exhibits a characteristic yellow color, allowing for easy detection and quantification of enzyme activity. This compound finds extensive applications in various biochemical and analytical techniques, including microbiological assays, enzyme-linked immunosorbent assays (ELISA), and histochemical staining methods.

• CAS Number: 74211-28-2