Utilized in a broad range of chemical manufacturing applications, the NucleoBond Xtra Midi Plus Kit for transfection-grade plasmid DNA is designed for the isolation and purification of high and low copy plasmid DNA from bacterial cultures. This gravity-based extraction kit features a version specifically tailored for midi preparations, ensuring efficient separation and purification within a preparation time of approximately 30 minutes per prep. The typical yield of this kit is 500 micrograms, with an endotoxin content ranging between 1 and 10 Endotoxin Units per microgram. The basic material used in this kit is E. The typical purity of the isolated plasmid DNA, as measured by the A260/A280 ratio, ranges from 1.8 to 1.95. This kit is stored at temperatures between 15 and 25 degrees Celsius.

• Category: Gravity-based extraction kits
• Typical yield: 500 µg
• Storage temp.: +15 to +25 °C
• Target: Plasmid DNA
• Automatic use: no
• Version: Midi prep
• Typical purity (A260/A280): 1.8−1.95
• Endotoxin content: 1−10 EU/µg
• Basic material: Bacteria, E. coli
• Quantity of material: < 200 ml (high copy), < 400 ml (low copy)
• Preparation time: 30 min/prep