Structured to assist in consistent experimental procedures, pre-column systems like the Hplc Guard Columns (Analytical) Nucleoshell ® Hilic play a crucial role in liquid chromatography (LC, HPLC). With a length of 4 mm and an inner diameter of 3 mm, these short columns are designed to protect the main HPLC column from contamination. Featuring core-shell, superficially porous particles (SPP), they have a particle size of 2.7 µm and a pore size of 90 Å. The surface chemistry is ammonium – sulfonic acid (zwitterionic) modified, making them suitable for a pH range of 2.0-8.5. They operate under a pressure of up to 600 bar and utilize the separation principle of Hydrophilic Interaction Chromatography (HILC).

• Category: Pre-column systems for HPLC
• Carbon content: 1.3 %
• Pressure stability: 600 bar
• pH stability: 2,0-8,5
• Eluent in column: Acetonitrile - Water
• Particle type: Core-shell, superficially porous particles (SPP)
• Column inner diameter: 3 mm
• L x Ø internal (column): 4 x 3 mm
• Pore size: 90 Å
• Specific surface according to BET: 130 m²/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Particle size: 2.7 µm
• Column length: 4 mm
• Endcapped: no