HPLC Guard Columns (analytical) NUCLEODUR ® NH 2

Commonly employed in multi-step synthesis and reaction processes, pre-column systems for high-performance liquid chromatography (HPLC) play a crucial role in ensuring efficient separation of compounds. One such system is the Nucleodur NH 2 HPLC guard column, belonging to the Analytical category. This guard column features fully porous particles (FPP) with a particle size of 5 µm, a column length of 4 mm, and an inner diameter of 3 mm. The column's pore size is 110 Å, providing ample space for analyte adsorption. The surface chemistry of the Nucleodur NH 2 column is aminopropyl (NH 2 ) modified, making it suitable for both normal phase (NP) and reversed phase (RP) separations. With a temperature stability of 60 °C and pressure stability up to 600 bar, this guard column can handle demanding conditions while maintaining its performance. The specific surface area, as determined by the Brunauer–Emmett–Teller (BET) method, is 340 m²/g. The eluent in the column is n-heptane, and the carbon content is 2.5 %. The pH stability range is 2.0 – 8.0, and the column has a pore volume of 0.9 ml/g. This guard column is an essential component in HPL.

• Category: Pre-column systems for HPLC
• Endcapped: yes
• Particle size: 5 µm
• L x Ø internal (column): 4 x 3 mm
• Eluent in column: n -Heptane
• Carbon content: 2.5 %
• Column inner diameter: 3 mm
• Column length: 4 mm