Structured to assist in consistent experimental procedures within the qPCR field, the Roti Lux Onestep RT-QPCR Master Mix is designed for probe-based real-time PCR assays. This low ROX version incorporates a HotStart DNA Polymerase, ensuring efficient amplification while maintaining specificity. The master mix includes all necessary components for reverse transcription, eliminating the need for additional steps when working with RNA templates. With an amplicon end of 3'dA, this versatile tool is ready for immediate use upon thawing at -20°C. Simply add primers and templates separately, and you're ready to proceed with your experiment. A single milliliter of the master mix is enough for one 96-well plate, making it a practical choice for high-throughput applications.

• Category: qPCR
• Basic material: RNA
• Density (D): 1 g/cm³
• Storage temp.: -20 °C
• Melting point (mp): 0 °C
• Polymerase: HotStart DNA Polymerase
• Application: Probe-based real-time PCR assays
• Amplicon ends: 3'dA
• Instructions for use, addition: The master mix contains all components for reverse transcription and qPCR. Only primer and template must be added separately. 1 ml Master Mix is sufficient for one 96-well plate.
• Boiling point (bp): 100 °C
• Version: low ROX