Supplied in compliance with established chemical handling standards, the Roti Lux Probe Qpcr Master Mix is designed for probe-based real-time PCR assays. This product features a modified HotStart DNA polymerase, dNTPs, MgCl2, and the necessary reaction buffer. For optimal results, users should add primers and templates separately. One milliliter of this master mix is sufficient for one 96-well plate, making it convenient for large-scale experiments. Store the mix at -20°C when not in use. The amplicon ends are 3'dA, ensuring efficient amplification.

• Category: qPCR
• Application: Probe-based real-time PCR assays
• Polymerase: HotStart DNA Polymerase
• Storage temp.: -20 °C
• Boiling point (bp): 100 °C
• Version: low ROX
• Basic material: DNA (cDNA / gDNA)
• Instructions for use, addition: The master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl 2 and the reaction buffer. Primer and template must be added separately. 1 ml Master Mix is sufficient for one 96-well plate.
• Amplicon ends: 3'dA