Crafted to support technical production environments, this Roti ® Lux Onestep RT-qPCR Master Mix is designed for probe-based real-time PCR assays. With its low ROX version and HotStart DNA Polymerase, it ensures efficient and accurate results. The master mix includes all components for reverse transcription, making it possible to use isolated RNA directly as starting material. This 5 mL product, divided into five 1 mL aliquots, is ideal for one 96-well plate, simplifying your workflow and reducing waste.

• Category: qPCR
• Storage temp.: -20 °C
• Amplicon ends: 3'dA
• Boiling point (bp): 100 °C
• Instructions for use, addition: The master mix contains all components for reverse transcription and qPCR. Only primer and template must be added separately. 1 ml Master Mix is sufficient for one 96-well plate.
• Application: Probe-based real-time PCR assays
• Version: low ROX
• Melting point (mp): 0 °C
• Basic material: RNA
• Polymerase: HotStart DNA Polymerase
• Density (D): 1 g/cm³