Formulated to support synthetic operations, the Hplc Column Nucleodur® Hilic, 5 μm, 50 mm, 3 mm offers a unique solution for separating highly polar analytes, such as amino acids, peptides, and water-soluble vitamins. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase, based on high-purity NUCLEODUR® silica gel, which provides an efficient strategy for modern analytics, particularly when reversed phase HPLC encounters limitations with polar compounds. The column's particle size of 5 μm ensures optimal performance under pressures up to 600 bar and temperatures up to 60°C. With a specific surface area of 340 m²/g, this column delivers exceptional results for a wide range of applications, including hydrophil

• Category: Separation columns for HPLC
• Phase: HILIC
• Specific surface according to BET: 340 m²/g
• Eluent in column: Acetonitrile - Water
• pH stability: 2,0-8,5
• Pore size: 110 Å
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• L x Ø internal (column): 50 x 3 mm
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Particle type: Fully porous particles (FPP)
• Column type: HPLC column
• Pore volume: 0.9 ml/g
• Column shape: Analytical separation column
• Pressure stability: 600 bar
• USP listing: Not available or proprietary
• Temperature stability: 60 ° C
• Endcapped: no
• Column volume: 0.35 ml
• Particle size: 5 µm