Widely applied in chemical workflows, separation columns for HPLC play an essential role in the purification and identification of various substances. One such column is the HPLC Column Nucleodur® C 4 Ec, 125 mm, 4 mm. This column features a medium density butyl modification, which is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules. Its particle size of 5 μm ensures efficient separation while maintaining a pressure stability of up to 450 bar. With a recommended application in biomolecular macromolecules, like proteins and peptides, this column offers a reliable solution for researchers in these fields.

• Category: Separation columns for HPLC
• Column volume: 1.56 ml
• Pore size: 300 Å
• L x Ø internal (column): 125 x 4 mm
• Column type: HPLC column
• Column shape: Analytical separation column
• Separation principle: Reversed phase (RP)
• Endcapped: yes
• Specific surface according to BET: 100 m²/g
• Pressure stability: 450 bar
• Pore volume: 0.9 ml/g
• USP listing: L26
• Temperature stability: 60 ° C
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Eluent in column: Acetonitrile - Water
• Similar phases: Jupiter ® C 4 ACE ® C 4
• pH stability: 1,0-9,0
• Particle size: 5 µm
• Phase: C 4 ec
• Particle type: Fully porous particles (FPP)
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions