Configured for stable performance in synthesis settings, this HPLC column Nucleodur® Hilic offers a unique solution for separating highly polar compounds. With its ammonium – sulfonic acid (zwitterionic) modification, it provides an efficient platform for the separation of amino acids, peptides, water-soluble vitamins, and other polar natural products. Its hydrophilic interactions and electrostatic retention mechanism make it ideal for handling complex mixtures, such as nucleosides, oligonucleotides, organic polar acids, and bases. This fully porous particle (FPP) column, featuring a specific surface area of 340 m²/g, boasts a pressure stability of 600 bar and temperature stability up to 60°C. It's designed for use with acetonitrile - water eluents and delivers a

• Category: Separation columns for HPLC
• Pore size: 110 Å
• Pressure stability: 600 bar
• Column shape: Analytical separation column
• Temperature stability: 60 ° C
• pH stability: 2,0-8,5
• Particle type: Fully porous particles (FPP)
• Particle size: 5 µm
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column volume: 1.25 ml
• Pore volume: 0.9 ml/g
• L x Ø internal (column): 100 x 4 mm
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Eluent in column: Acetonitrile - Water
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Endcapped: no
• Column type: HPLC column
• Specific surface according to BET: 340 m²/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Phase: HILIC
• USP listing: Not available or proprietary