Engineered to maintain reliability in process-driven workflows, Macherey-Nagel's Separation columns for HPLC, such as the HPLC Column Nucleodur® Phenyl-Hexyl, 5 μm, 250 mm, 4.6 mm, are designed to deliver consistent results. Featuring a Phenyl-Hexyl phase, these columns offer an alternative selectivity compared to traditional C18 modifications due to their dual retention mechanisms based on π-π interactions and hydrophobic interactions. With a pH stability range of 1.0-10.0, they are suitable for a wide variety of applications including antibiotics, aromatic and unsaturated compounds, pharmaceutical products, steroids, and even LC-MS. Additionally, these columns exhibit low bleeding characteristics, making them ideal for use in LC-MS

• Category: Separation columns for HPLC
• Recommended application(s): Antibiotics Aromatic and unsaturated compounds Suitable for LC-MS Pharmaceutical products Especially for polar compounds Steroids
• Pressure stability: 600 bar
• Pore size: 110 Å
• Interactions / retention mechanism: π-π and hydrophobic
• Separation principle: Reversed phase (RP)
• Particle type: Fully porous particles (FPP)
• Temperature stability: 60 ° C
• USP listing: L11
• Column volume: 4.15 ml
• Particle size: 5 µm
• Pore volume: 0.9 ml/g
• Column shape: Analytical separation column
• L x Ø internal (column): 250 x 4.6 mm
• Application Database (Macherey-Nagel): HPLC
• Eluent in column: Acetonitrile - Water
• Surface chemistry: Phenylhexyl modification
• Endcapped: yes
• pH stability: 1,0-10,0
• Specific surface according to BET: 340 m²/g
• Phase: Phenyl-Hexyl
• Column type: HPLC column