Developed for demanding processing applications, the HPLC column Nucleodur® C 18 ec offers exceptional performance for separating biomolecules such as proteins and peptides. This analytical separation column features a particle size of 5 μm, a specific surface area of 340 m²/g, and a pore size of 300 Å, ensuring efficient separation while maintaining high resolution. Its medium density monomeric octadecyl (C 18, ODS) modification provides hydrophobic interactions and some residual silanol interactions, making it ideal for a wide range of applications. With a pressure stability of 450 bar and temperature stability up to 60°C, this column is designed to handle even the most challenging conditions.

• Category: Separation columns for HPLC
• Column type: HPLC column
• Eluent in column: Acetonitrile - Water
• Surface chemistry: Medium density monomeric octadecyl (C 18 , ODS) modification
• Particle size: 5 µm
• USP listing: L1
• Pressure stability: 450 bar
• Specific surface according to BET: 340 m²/g
• Phase: C 18 ec
• Separation principle: Reversed phase (RP)
• Pore size: 300 Å
• L x Ø internal (column): 100 x 4.6 mm
• Pore volume: 0.9 ml/g
• Similar phases: NUCLEOSIL ® C 18 Spherisorb ® ODS II Symmetry ® C 18 Hypersil™ ODS Inertsil ® ODS II Kromasil ® C 18 LiChrospher ® RP 18
• Column volume: 1.65 ml
• Temperature stability: 60 ° C
• Particle type: Fully porous particles (FPP)
• pH stability: 1,0-9,0
• Column shape: Analytical separation column
• Endcapped: yes
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions