Widely applied in chemical workflows, the HPLC column NUCLEODUR® PFP, 5 μm, 50 mm, 3 mm, falls under the category of separation columns for High Performance Liquid Chromatography (HPLC). This particular column features a pentafluorophenyl-propyl modification on high purity NUCLEODUR® silica gel, offering an alternative selectivity compared to traditional C18 modifications. Its separation mechanism is based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions, and hydrophobic interactions. Due to its low bleeding characteristics, it is suitable for LC/MS applications. It comes with an analytical HPLC column, instruction leaflet, and certificate of analysis.

• Category: Separation columns for HPLC
• Column type: HPLC column
• USP listing: L43
• Interactions / retention mechanism: polar (H-bond), dipole-dipole, π-π and hydrophobic
• Pore size: 110 Å
• Recommended application(s): Antibiotics Aromatic and unsaturated compounds Basic compounds Suitable for LC-MS Halogenated compounds Isomers Pharmaceutical products Phenols Especially for polar compounds
• Column volume: 0.35 ml
• Pore volume: 0.9 ml/g
• Phase: PFP
• Particle size: 5 µm
• Column shape: Analytical separation column
• Particle type: Fully porous particles (FPP)
• Specific surface according to BET: 340 m²/g
• Endcapped: yes
• pH stability: 1,0-9,0
• Eluent in column: Acetonitrile - Water
• Pressure stability: 600 bar
• Separation principle: Reversed phase (RP)
• Temperature stability: 60 ° C
• Surface chemistry: Pentafluorophenylpropyl (PFP) modification
• L x Ø internal (column): 50 x 3 mm