Produced to ensure dependable processing results, this HPLC column Nucleodur® Hilic offers a reliable solution for separating highly polar compounds. With a particle size of 5 microns and a pore size of 110 angstroms, it provides a large surface area for efficient interactions. Its unique ammonium – sulfonic acid (zwitterionic) modification enables hydrophilic interaction chromatography (HILC), making it ideal for separating amino acids, peptides, nucleosides, oligonucleotides, organic polar acids and bases, water-soluble vitamins, and other polar natural products. This column's temperature stability up to 60°C and pressure resistance up to 600 bar further enhance its versatility in various analytical applications.

• Category: Separation columns for HPLC
• Specific surface according to BET: 340 m²/g
• Pore size: 110 Å
• Column volume: 2.06 ml
• Endcapped: no
• Pore volume: 0.9 ml/g
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column type: HPLC column
• Particle type: Fully porous particles (FPP)
• Column shape: Analytical separation column
• pH stability: 2,0-8,5
• L x Ø internal (column): 125 x 4.6 mm
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Temperature stability: 60 ° C
• Pressure stability: 600 bar
• Particle size: 5 µm
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• USP listing: Not available or proprietary
• Eluent in column: Acetonitrile - Water
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Phase: HILIC