Recommended for applications requiring controlled solvent behavior, such as the separation of biomolecular macromolecules like proteins and peptides, the HPLC column Nucleodur® C 18 Ec, 5 μm, 300 Å, 250 mm, 2 mm offers excellent performance. This fully porous particle (FPP) column features a medium density monomeric octadecyl (C 18, ODS) modification, ensuring hydrophobic (van der Waals interactions) and some residual silanol interactions for optimal retention mechanisms. With a specific surface area of 340 m²/g according to BET, this analytical separation column delivers a column volume of 0.8 ml and a pressure stability of 450 bar, making it suitable for temperatures up to 60°C.

• Category: Separation columns for HPLC
• Column shape: Analytical separation column
• pH stability: 1,0-9,0
• Pore size: 300 Å
• USP listing: L1
• Application Database (Macherey-Nagel): HPLC
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Separation principle: Reversed phase (RP)
• Pore volume: 0.9 ml/g
• L x Ø internal (column): 250 x 2 mm
• Particle size: 5 µm
• Endcapped: yes
• Temperature stability: 60 ° C
• Particle type: Fully porous particles (FPP)
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Surface chemistry: Medium density monomeric octadecyl (C 18 , ODS) modification
• Column type: HPLC column
• Phase: C 18 ec
• Specific surface according to BET: 340 m²/g
• Eluent in column: Acetonitrile - Water
• Similar phases: NUCLEOSIL ® C 18 Spherisorb ® ODS II Symmetry ® C 18 Hypersil™ ODS Inertsil ® ODS II Kromasil ® C 18 LiChrospher ® RP 18
• Column volume: 0.8 ml
• Pressure stability: 450 bar