Crafted to support technical production environments, the HPLC column Nucleodur® C 18 Isis, featuring a 5 μm particle size and a length of 250 mm, is designed for analytical separations. This column's unique nonpolar crosslinked polymeric octadecyl (C 18, ODS) modification offers high steric selectivity, making it ideal for separating positional and structural isomers, planar/non-planar molecules, steroids, fat-soluble vitamins, and even (o,p,m)-substituted aromatics. With a recommended operating temperature of up to 60°C and pressure stability of 600 bar, this column ensures robust performance under demanding conditions. It also boasts exceptional compatibility with LC-MS applications.

• Category: Separation columns for HPLC
• Column type: HPLC column
• Endcapped: yes
• pH stability: 1,0-10,0
• Particle size: 5 µm
• Pore volume: 0.9 ml/g
• Recommended application(s): Separation of positional and structural isomers Planar/non-planar molecules Steroids Suitable for LC-MS (o,p,m-)Substituted aromatics Fat-soluble vitamins Exceptional steric selectivity
• Interactions / retention mechanism: steric and hydrophobic
• USP listing: L1
• Pressure stability: 600 bar
• Pore size: 110 Å
• Particle type: Fully porous particles (FPP)
• L x Ø internal (column): 250 x 4.6 mm
• Application Database (Macherey-Nagel): HPLC
• Specific surface according to BET: 340 m²/g
• Column shape: Analytical separation column
• Surface chemistry: Nonpolar crosslinked polymeric octadecyl (C 18 , ODS) modification, High steric selectivity
• Eluent in column: Acetonitrile - Water
• Temperature stability: 60 ° C
• Phase: C 18 Isis
• Separation principle: Reversed phase (RP)
• Column volume: 4.15 ml