Structured to assist in consistent experimental procedures, the Hplc Column Nucleodur ® NH 2 -RP, 5 μm, 150 mm, 4.6 mm, offers versatile applications due to its polar aminopropyl modification. This column is suitable for separating polar compounds under reversed-phase (RP) conditions, such as sugars and DNA bases, as well as hydrocarbons under normal-phase (NP) conditions. With a pH stability range of 2.0-8.0 and temperature stability up to 60°C, this column ensures reliable results within a wide range of experimental parameters. Additionally, it features a specific surface area of 340 m²/g, ensuring efficient separation through multi-mode interactions including polar/ionic and hydrophobic mechanisms.

• Category: Separation columns for HPLC
• Similar phases: NUCLEOSIL ® NH 2 / NH 2 -RP
• L x Ø internal (column): 150 x 4.6 mm
• Recommended application(s): Polar compounds under RP conditions (sugars, DNA bases) Hydrocarbons under NP conditions
• Particle type: Fully porous particles (FPP)
• Column shape: Analytical separation column
• Column type: HPLC column
• Separation principle: Multi-mode (NP and RP), Reversed phase (RP)
• USP listing: L8
• pH stability: 2,0-8,0
• Phase: NH 2 -RP
• Eluent in column: Acetonitrile - Water
• Interactions / retention mechanism: polar/ionic and hydrophobic
• Pore size: 110 Å
• Column volume: 2.5 ml
• Temperature stability: 60 ° C
• Specific surface according to BET: 340 m²/g
• Endcapped: no
• Particle size: 5 µm
• Pressure stability: 600 bar
• Pore volume: 0.9 ml/g
• Surface chemistry: Aminopropyl (NH 2 ) modification