Suitable for laboratory and industrial environments, the HPLC Column Nucleoshell® Hilic, 50 mm, 4 mm offers a solution for separating highly polar analytes such as amino acids, water-soluble vitamins, and organic polar acids and bases. This core-shell, superficially porous particle (SPP) column features a zwitterionic ammonium – sulfonic acid surface chemistry and operates using the hydrophilic interaction chromatography (HILIC) principle. With a pore size of 90 Å, a specific surface area of 130 m²/g, and a pressure stability of up to 600 bar, this analytical separation column is ideal for applications like peptide and oligonucleotide separations, and it's compatible with LC-MS.

• Category: Separation columns for HPLC
• Particle size: 2.7 µm
• Recommended application(s): Amino acids Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural substances Water-soluble vitamins Suitable for LC-MS
• Temperature stability: 60 ° C
• Pore volume: 0.9 ml/g
• Pressure stability: 600 bar
• pH stability: 2,0-8,5
• Specific surface according to BET: 130 m²/g
• L x Ø internal (column): 50 x 4 mm
• Particle type: Core-shell, superficially porous particles (SPP)
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Endcapped: no
• Phase: HILIC
• Column type: HPLC column
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• USP listing: Not available or proprietary
• Eluent in column: Acetonitrile - Water
• Column volume: 0.62 ml
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Application Database (Macherey-Nagel): HPLC
• Column shape: Analytical separation column
• Pore size: 90 Å