Engineered for consistent performance, this HPLC column Nucleodur® C 18 ec is designed specifically for the separation of biomolecules such as proteins and peptides. Featuring a particle size of 5 μm, 300 Å pore size, and a specific surface area of 340 m²/g according to BET, it offers excellent separation efficiency. The column's medium density monomeric octadecyl (C 18, ODS) modification ensures hydrophobic interactions and some residual silanol interactions, making it ideal for a wide range of applications. With a pressure stability of 450 bar and temperature stability up to 60°C, this analytical separation column is robust enough for daily routine analyses and upscaling for preparative HPLC.

• Category: Separation columns for HPLC
• Surface chemistry: Medium density monomeric octadecyl (C 18 , ODS) modification
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Pore size: 300 Å
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• USP listing: L1
• Separation principle: Reversed phase (RP)
• Eluent in column: Acetonitrile - Water
• Temperature stability: 60 ° C
• Particle size: 5 µm
• pH stability: 1,0-9,0
• Endcapped: yes
• Pressure stability: 450 bar
• L x Ø internal (column): 100 x 2 mm
• Phase: C 18 ec
• Column shape: Analytical separation column
• Column volume: 0.3 ml
• Similar phases: NUCLEOSIL ® C 18 Spherisorb ® ODS II Symmetry ® C 18 Hypersil™ ODS Inertsil ® ODS II Kromasil ® C 18 LiChrospher ® RP 18
• Pore volume: 0.9 ml/g
• Particle type: Fully porous particles (FPP)
• Column type: HPLC column
• Specific surface according to BET: 340 m²/g