Widely applied in chemical workflows, the Hplc Column Nucleodur ® Hilic, 3 μm, 50 mm, 4 mm belongs to the category of Separation columns for HPLC. This analytical separation column features a particle size of 3 μm, a pore size of 110 Å, and a specific surface area of 340 m²/g according to BET. Its surface chemistry is ammonium – sulfonic acid (zwitterionic) modified, making it suitable for the separation of highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. The column operates under Hydrophilic Interaction Chromatography (HILIC) principles, with recommended eluents being acetonitrile - water mixtures. It offers pressure stability

• Category: Separation columns for HPLC
• pH stability: 2,0-8,5
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Pressure stability: 600 bar
• Column volume: 0.62 ml
• Application Database (Macherey-Nagel): HPLC
• Temperature stability: 60 ° C
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column type: HPLC column
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Specific surface according to BET: 340 m²/g
• Particle type: Fully porous particles (FPP)
• Phase: HILIC
• Particle size: 3 µm
• USP listing: Not available or proprietary
• Endcapped: no
• Pore size: 110 Å
• Pore volume: 0.9 ml/g
• L x Ø internal (column): 50 x 4 mm
• Eluent in column: Acetonitrile - Water
• Column shape: Analytical separation column
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins