HPLC column NUCLEODUR ® C 18 Pyramid, 5 µm, 250 mm, 4 mm

Built to deliver predictable results in synthesis routines, the HPLC Column Nucleodur ® C 18 Pyramid offers a unique combination of particle size, pore volume, and specific surface area, ensuring optimal performance in reversed-phase separations. With a particle size of 5 μm, this fully porous particle (FPP) column provides excellent efficiency and resolution while maintaining high pressure stability up to 600 bar. Its polar endcapping makes it suitable for aqueous eluents, making it an ideal choice for separating very polar compounds such as pharmaceutical raw materials, organic acids, and water-soluble vitamins. This column's temperature stability of 60°C further enhances its versatility, allowing for a wide range of applications including complexing agents, nucleic acid bases, penicillin antibiotics, and more. Available in

• Category: Separation columns for HPLC
• Phase: C 18 Pyramid
• Specific surface according to BET: 340 m²/g
• Recommended application(s): Pharmaceutical raw materials Very polar compounds Analgesics Suitable for 100 % aqueous eluents Water-soluble vitamins Complexing agents Nucleic acid bases Organic acids Penicillin antibiotics
• Separation principle: Reversed phase (RP)
• Pressure stability: 600 bar
• Pore volume: 0.9 ml/g
• Pore size: 110 Å
• pH stability: 1,0-9,0
• Application Database (Macherey-Nagel): HPLC
• Column shape: Analytical separation column
• L x Ø internal (column): 250 x 4 mm
• Temperature stability: 60 ° C
• Eluent in column: Acetonitrile - Water
• Particle size: 5 µm
• Interactions / retention mechanism: hydrophobic and polar (H bonds)
• Endcapped: yes
• Surface chemistry: Octadecyl (C 18 , ODS) modification, Polar endcapping
• USP listing: L1
• Column type: HPLC column
• Column volume: 3.15 ml
• Similar phases: Aqua ® Synergi™ Hydro-RP AQ Atlantis ® dC 18 Polaris ® C18-A
• Particle type: Fully porous particles (FPP)