Tailored for precision-driven chemical tasks, these Separation columns for HPLC, specifically the Hplc Column Nucleodur ® Hilic, 5 μm, 100 Mm, 2 Mm, are designed to cater to the needs of modern analytics, particularly for polar compounds where reversed phase HPLC may be limited. This column features a zwitterionic HILIC phase based on high purity NUCLEODUR ® silica gel, offering an additional tool for separating polar analytes in HPLC. With a particle size of 5 μm, it boasts a pressure stability of 600 bar and temperature stability up to 60°C, making it suitable for various applications such as amino acids, highly polar analytes, organic polar acids and bases, peptides, and water-soluble vitamins. Its specific

• Category: Separation columns for HPLC
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Endcapped: no
• Phase: HILIC
• Pore volume: 0.9 ml/g
• Column shape: Analytical separation column
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• USP listing: Not available or proprietary
• Particle size: 5 µm
• Column volume: 0.3 ml
• L x Ø internal (column): 100 x 2 mm
• Particle type: Fully porous particles (FPP)
• Pressure stability: 600 bar
• Temperature stability: 60 ° C
• pH stability: 2,0-8,5
• Column type: HPLC column
• Pore size: 110 Å
• Eluent in column: Acetonitrile - Water
• Application Database (Macherey-Nagel): HPLC
• Specific surface according to BET: 340 m²/g