HPLC column NUCLEODUR ® HILIC, 3 µm, 50 mm, 4.6 mm

Designed with efficiency in synthetic processes in mind, the Hplc Column Nucleodur ® Hilic, 3 μm, 50 Mm, 4.6 Mm offers a unique solution for separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase, based on high purity NUCLEODUR ® silica gel, which provides an alternative strategy for modern analytics, particularly when reversed phase HPLC encounters limitations with polar compounds. With a pH stability range of 2.0-8.5, temperature stability up to 60°C, and pressure stability of 600 bar, this column ensures robust performance even under demanding conditions. Its specific surface area of 3

• Category: Separation columns for HPLC
• Particle type: Fully porous particles (FPP)
• Pore size: 110 Å
• Endcapped: no
• Column volume: 0.83 ml
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Pore volume: 0.9 ml/g
• Pressure stability: 600 bar
• Eluent in column: Acetonitrile - Water
• L x Ø internal (column): 50 x 4.6 mm
• Phase: HILIC
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• pH stability: 2,0-8,5
• Temperature stability: 60 ° C
• Column shape: Analytical separation column
• Particle size: 3 µm
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Column type: HPLC column
• Specific surface according to BET: 340 m²/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• USP listing: Not available or proprietary