HPLC column NUCLEODUR ® C 4 ec, 125 mm, 3 mm

Developed to align with modern chemical standards, the Hplc Column Nucleodur ® C 4 Ec, 125 Mm, 3 Mm falls under the category of Separation columns for HPLC. This particular column features a medium density butyl modification, which is based on high purity NUCLEODUR ® silica gel and is commonly used for the separation of biomolecules such as proteins and peptides. With a particle size of 5 µm and a specific surface area of 100 m²/g according to BET, this column offers efficient separation capabilities while maintaining a pressure stability of up to 450 bar and temperature stability of 60 °C. The column's recommended eluent is acetonitrile-water, making it suitable for various applications within the field of liquid chromatography.

• Category: Separation columns for HPLC
• Specific surface according to BET: 100 m²/g
• Similar phases: Jupiter ® C 4 ACE ® C 4
• Pressure stability: 450 bar
• Pore size: 300 Å
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Particle type: Fully porous particles (FPP)
• Particle size: 5 µm
• L x Ø internal (column): 125 x 3 mm
• Phase: C 4 ec
• Endcapped: yes
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Column volume: 0.87 ml
• pH stability: 1,0-9,0
• USP listing: L26
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Column shape: Analytical separation column
• Separation principle: Reversed phase (RP)
• Eluent in column: Acetonitrile - Water
• Column type: HPLC column
• Pore volume: 0.9 ml/g
• Temperature stability: 60 ° C