HPLC column NUCLEODUR ® HILIC, 5 µm, 250 mm, 4.6 mm

Designed with efficiency in synthetic processes in mind, this HPLC column Nucleodur® Hilic, featuring a particle size of 5 μm and a length of 250 mm with an inner diameter of 4.6 mm, is ideal for separating highly polar analytes such as amino acids, peptides, and water-soluble vitamins. Constructed using fully porous particles (FPP), it boasts a specific surface area of 340 m²/g and a pore size of 110 Å, ensuring optimal interaction and retention mechanisms through its ammonium – sulfonic acid (zwitterionic) modified surface. This column operates effectively within a temperature range of 60°C and pressure stability up to 600 bar, making it suitable for various HILIC applications in your chromatography lab.

• Category: Separation columns for HPLC
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Temperature stability: 60 ° C
• Specific surface according to BET: 340 m²/g
• L x Ø internal (column): 250 x 4.6 mm
• Column volume: 4.15 ml
• Pore volume: 0.9 ml/g
• Particle type: Fully porous particles (FPP)
• Particle size: 5 µm
• Column shape: Analytical separation column
• pH stability: 2,0-8,5
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Pore size: 110 Å
• Eluent in column: Acetonitrile - Water
• Column type: HPLC column
• USP listing: Not available or proprietary
• Pressure stability: 600 bar
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Endcapped: no
• Phase: HILIC
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)