Widely applied in chemical workflows, separation columns for HPLC play a crucial role in the identification and quantification of various compounds. One such column is the HPLC column NUCLEODUR® PFP, featuring a pentafluorophenyl-propyl modification that offers an alternative selectivity compared to traditional C18 modifications. This reversed-phase column is suitable for LC/MS applications due to its low bleeding characteristics and operates under a pressure of up to 600 bar at temperatures as high as 60°C. With a specific surface area of 340 m²/g and a particle size of 5 μm, it exhibits four retention mechanisms—polar interactions (H bonds), dipole-dipole interactions, π-π interactions, and hydrophobic interactions—making it particularly effective for separating polar compounds like antibiotics, arom

• Category: Separation columns for HPLC
• Surface chemistry: Pentafluorophenylpropyl (PFP) modification
• Separation principle: Reversed phase (RP)
• Pore size: 110 Å
• Eluent in column: Acetonitrile - Water
• Particle size: 5 µm
• Recommended application(s): Antibiotics Aromatic and unsaturated compounds Basic compounds Suitable for LC-MS Halogenated compounds Isomers Pharmaceutical products Phenols Especially for polar compounds
• Column volume: 2.06 ml
• Interactions / retention mechanism: polar (H-bond), dipole-dipole, π-π and hydrophobic
• Endcapped: yes
• Phase: PFP
• Particle type: Fully porous particles (FPP)
• Pore volume: 0.9 ml/g
• Pressure stability: 600 bar
• Column type: HPLC column
• USP listing: L43
• Temperature stability: 60 ° C
• L x Ø internal (column): 125 x 4.6 mm
• Column shape: Analytical separation column
• Specific surface according to BET: 340 m²/g
• pH stability: 1,0-9,0