Configured for stable performance in synthesis settings, the HPLC Column Nucleodur® Hilic, 3 μm, 250 mm, 2 mm offers a unique solution for separating highly polar analytes such as amino acids, peptides, and water-soluble vitamins. This analytical separation column features a zwitterionic HILIC phase based on high purity NUCLEODUR® silica gel, providing excellent separation capabilities for polar and ionic compounds under RP conditions. With a pH stability range of 2.0-8.5, temperature stability up to 60°C, and pressure stability of 600 bar, this column ensures reliable results even under demanding conditions.

• Category: Separation columns for HPLC
• Pressure stability: 600 bar
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Temperature stability: 60 ° C
• Column type: HPLC column
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Particle type: Fully porous particles (FPP)
• Particle size: 3 µm
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• pH stability: 2,0-8,5
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Endcapped: no
• Column shape: Analytical separation column
• Pore size: 110 Å
• USP listing: Not available or proprietary
• Eluent in column: Acetonitrile - Water
• Specific surface according to BET: 340 m²/g
• Column volume: 0.8 ml
• Phase: HILIC
• Pore volume: 0.9 ml/g
• L x Ø internal (column): 250 x 2 mm