Designed with efficiency in synthetic processes in mind, this HPLC column Nucleodur® Hilic, featuring a particle size of 3 μm and a length of 125 mm, is ideal for separating highly polar analytes such as amino acids, peptides, and water-soluble vitamins. Its unique zwitterionic surface chemistry, based on high purity NUCLEODUR® silica gel, allows for hydrophilic interaction chromatography (HILIC), making it particularly suitable for separating polar and ionic compounds under reversed phase conditions. With a pH stability range of 2.0-8.5 and pressure stability up to 600 bar, this column ensures reliable performance even under demanding conditions.

• Category: Separation columns for HPLC
• Application Database (Macherey-Nagel): HPLC
• Eluent in column: Acetonitrile - Water
• Column type: HPLC column
• Particle type: Fully porous particles (FPP)
• Phase: HILIC
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Pressure stability: 600 bar
• Pore volume: 0.9 ml/g
• Column shape: Analytical separation column
• L x Ø internal (column): 125 x 4 mm
• Specific surface according to BET: 340 m²/g
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Pore size: 110 Å
• Column volume: 1.56 ml
• Particle size: 3 µm
• pH stability: 2,0-8,5
• Temperature stability: 60 ° C
• USP listing: Not available or proprietary
• Endcapped: no
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification