Optimized for controlled laboratory processes, the HPLC Column Nucleodur® Hilic, 5 μm, 125 mm, 3 mm offers a solution for the separation of highly polar analytes. This column features a zwitterionic HILIC phase based on high purity NUCLEODUR® silica gel, designed for amino acids, peptides, water-soluble vitamins, and other polar natural products. With a pH stability range of 2.0-8.5, it ensures versatile applicability. The column's particle size of 5 μm, combined with its pressure stability up to 600 bar and temperature stability at 60°C, makes it suitable for various experimental conditions. Recommended eluents include acetonitrile-water mixtures, ensuring efficient separations in Hydrophilic Interaction Chrom

• Category: Separation columns for HPLC
• Pore volume: 0.9 ml/g
• L x Ø internal (column): 125 x 3 mm
• Specific surface according to BET: 340 m²/g
• pH stability: 2,0-8,5
• Particle type: Fully porous particles (FPP)
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column type: HPLC column
• Particle size: 5 µm
• Eluent in column: Acetonitrile - Water
• Phase: HILIC
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Endcapped: no
• Pore size: 110 Å
• USP listing: Not available or proprietary
• Column shape: Analytical separation column
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Column volume: 0.87 ml
• Pressure stability: 600 bar
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Temperature stability: 60 ° C