Produced to ensure dependable processing results, this HPLC column features a C 18 ec phase with a particle size of 5 μm and a pore size of 300 Å. Its specific surface area, as per BET, is 340 m²/g, making it ideal for separating biomolecular macromolecules such as proteins and peptides. With a recommended temperature stability of 60 °C and pressure resistance up to 450 bar, this column is designed for robust performance under various conditions. It comes equipped with fully porous particles (FPP) and exhibits hydrophobic interactions, along with some residual silanol interactions, ensuring versatile retention mechanisms for diverse applications. This analytical separation column is delivered with an instruction leaflet, certificate of analysis, and is compatible with eluents such as acetonitrile and water.

• Category: Separation columns for HPLC
• Eluent in column: Acetonitrile - Water
• Separation principle: Reversed phase (RP)
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Particle size: 5 µm
• Column shape: Analytical separation column
• Phase: C 18 ec
• Endcapped: yes
• USP listing: L1
• Pore volume: 0.9 ml/g
• Pressure stability: 450 bar
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Column type: HPLC column
• Surface chemistry: Medium density monomeric octadecyl (C 18 , ODS) modification
• pH stability: 1,0-9,0
• Application Database (Macherey-Nagel): HPLC
• Column volume: 4.15 ml
• Specific surface according to BET: 340 m²/g
• L x Ø internal (column): 250 x 4.6 mm
• Similar phases: NUCLEOSIL ® C 18 Spherisorb ® ODS II Symmetry ® C 18 Hypersil™ ODS Inertsil ® ODS II Kromasil ® C 18 LiChrospher ® RP 18
• Pore size: 300 Å
• Particle type: Fully porous particles (FPP)
• Temperature stability: 60 ° C