Prepared for use in regulated laboratory conditions, the HPLC Column Nucleodur ® Hilic, 3 μm, 150 mm, 3 mm features a hydrophilic interaction chromatography (HILC) separation principle and ammonium – sulfonic acid (zwitterionic) surface chemistry. This analytical separation column has a specific surface area of 340 m²/g as determined by BET, a pore size of 110 Å, and a pore volume of 0.9 ml/g. It is designed for use with fully porous particles (FPP), with a particle size of 3 μm and a pressure stability of up to 600 bar. The recommended applications include amino acids, highly polar analytes, hydrophilic compounds, nucleosides, oligonucleotides, organic polar ac

• Category: Separation columns for HPLC
• Specific surface according to BET: 340 m²/g
• Temperature stability: 60 ° C
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Particle size: 3 µm
• Column volume: 1.05 ml
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• L x Ø internal (column): 150 x 3 mm
• USP listing: Not available or proprietary
• Particle type: Fully porous particles (FPP)
• pH stability: 2,0-8,5
• Pressure stability: 600 bar
• Pore volume: 0.9 ml/g
• Phase: HILIC
• Column type: HPLC column
• Eluent in column: Acetonitrile - Water
• Column shape: Analytical separation column
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Endcapped: no
• Pore size: 110 Å