Produced to ensure dependable processing results, the Hplc Column Nucleodur ® Polartec, 3 μm, 250 mm, 2 mm offers exceptional versatility in separating various compounds such as amino acids, basic pharmaceuticals, nitrogen containing compounds, organic acids, pesticides, phenols, water-soluble vitamins, and even suitable for 100% aqueous eluents. With its unique combination of a polar group embedded in a hydrophobic C 18 modification, this HPLC phase exhibits both steric and enhanced polar selectivity, making it ideal for use under high aqueous conditions. Furthermore, its hydrophobic nature with pronounced hydrophilic properties ensures optimal performance in liquid chromatography (LC, HPLC). The column's specifications include a particle size of 3

• Category: Separation columns for HPLC
• Surface chemistry: Octadecyl phase (C 18 , ODS) modification, with embedded polar group (PEG)
• Separation principle: Reversed phase (RP)
• pH stability: 1,0-9,0
• Temperature stability: 60 ° C
• Interactions / retention mechanism: hydrophobic and polar (H bonds)
• Eluent in column: Acetonitrile - Water
• Endcapped: yes
• Column volume: 0.8 ml
• Particle size: 3 µm
• Phase: PolarTec
• L x Ø internal (column): 250 x 2 mm
• USP listing: L1 L60
• Pressure stability: 600 bar
• Pore size: 110 Å
• Column shape: Analytical separation column
• Particle type: Fully porous particles (FPP)
• Specific surface according to BET: 340 m²/g
• Recommended application(s): Amino acids Basic pharmaceuticals Especially for polar compounds Nitrogen containing compounds Organic acids Pesticides Phenols Suitable for 100 % aqueous eluents Suitable for LC-MS Water-soluble vitamins
• Application Database (Macherey-Nagel): HPLC
• Pore volume: 0.9 ml/g
• Column type: HPLC column