Tailored for precision-driven chemical tasks, our Separation columns for HPLC include the Hplc Column Nucleodur ® Hilic, 5 μm, 250 mm, 3 mm. This analytical HPLC column features a hydrophilic interaction chromatography (HILIC) phase, based on high purity NUCLEODUR ® silica gel, designed for separating polar analytes in HPLC. With a pH stability range of 2.0-8.5 and temperature stability up to 60°C, it offers excellent performance for amino acids, highly polar analytes, organic polar acids and bases, peptides, polar natural products, water-soluble vitamins, and other hydrophilic compounds. The column's specific surface area, as per BET, is 340 m²/g

• Category: Separation columns for HPLC
• Pressure stability: 600 bar
• Eluent in column: Acetonitrile - Water
• Pore volume: 0.9 ml/g
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Pore size: 110 Å
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Phase: HILIC
• Column type: HPLC column
• Particle size: 5 µm
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Specific surface according to BET: 340 m²/g
• Column shape: Analytical separation column
• Particle type: Fully porous particles (FPP)
• pH stability: 2,0-8,5
• L x Ø internal (column): 250 x 3 mm
• Column volume: 1.75 ml
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• USP listing: Not available or proprietary
• Temperature stability: 60 ° C
• Endcapped: no