Engineered for consistent performance, this HPLC column Nucleodur® C 18 Isis features a 75mm x 4.6mm column with a 5μm particle size, offering a high specific surface area of 340 m²/g. Its nonpolar crosslinked polymeric octadecyl (C 18, ODS) modification ensures exceptional steric selectivity, making it suitable for separating positional and structural isomers, planar/non-planar molecules, steroids, fat-soluble vitamins, and even (o,p,m)-substituted aromatics. With a recommended eluent of acetonitrile-water, this column is compatible with LC-MS applications and boasts a pressure stability of up to 600 bar and temperature stability of 60°C.

• Category: Separation columns for HPLC
• Pore volume: 0.9 ml/g
• Eluent in column: Acetonitrile - Water
• Separation principle: Reversed phase (RP)
• Particle type: Fully porous particles (FPP)
• Column shape: Analytical separation column
• Column volume: 1.25 ml
• Phase: C 18 Isis
• Interactions / retention mechanism: steric and hydrophobic
• Temperature stability: 60 ° C
• Pressure stability: 600 bar
• USP listing: L1
• Endcapped: yes
• Particle size: 5 µm
• Pore size: 110 Å
• Specific surface according to BET: 340 m²/g
• pH stability: 1,0-10,0
• L x Ø internal (column): 75 x 4.6 mm
• Column type: HPLC column
• Recommended application(s): Separation of positional and structural isomers Planar/non-planar molecules Steroids Suitable for LC-MS (o,p,m-)Substituted aromatics Fat-soluble vitamins Exceptional steric selectivity
• Application Database (Macherey-Nagel): HPLC
• Surface chemistry: Nonpolar crosslinked polymeric octadecyl (C 18 , ODS) modification, High steric selectivity