Prepared to meet the demands of technical research tasks, this HPLC column Nucleodur® Hilic offers a solution for separating highly polar analytes that may be challenging using traditional reversed-phase HPLC methods. With a particle size of 5 μm and a pore size of 110 Å, it provides a large surface area for efficient interactions between the analyte and the stationary phase. Its unique ammonium – sulfonic acid (zwitterionic) modification allows for ionic and hydrophilic interactions, making it ideal for separating a wide range of polar compounds such as amino acids, nucleosides, oligonucleotides, organic polar acids and bases, peptides, and water-soluble vitamins. This HPLC column is designed to operate at temperatures up to 60°C and pressures up to 600

• Category: Separation columns for HPLC
• Column shape: Analytical separation column
• USP listing: Not available or proprietary
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Phase: HILIC
• Column volume: 0.62 ml
• Specific surface according to BET: 340 m²/g
• Particle type: Fully porous particles (FPP)
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Pore volume: 0.9 ml/g
• Endcapped: no
• Particle size: 5 µm
• pH stability: 2,0-8,5
• Temperature stability: 60 ° C
• Column type: HPLC column
• Pressure stability: 600 bar
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Pore size: 110 Å
• L x Ø internal (column): 50 x 4 mm
• Eluent in column: Acetonitrile - Water