Optimized for controlled laboratory processes, this HPLC column from Nucleodur features a HILIC phase, making it ideal for separating polar compounds that may prove challenging using traditional reversed-phase methods. With a particle size of 1.8 microns, this UHPLC column offers excellent pressure stability up to 900 bar and temperature stability up to 60 degrees Celsius. Its ammonium – sulfonic acid (zwitterionic) modification ensures strong interactions with both ionic and hydrophilic compounds, while its specific surface area of 340 square meters per gram provides ample opportunity for these interactions to occur. This column is suitable for a wide range of applications, including amino acids, peptides, water-soluble vitamins, and highly polar analytes such as nucleosides and oligonucleotides.

• Category: Separation columns for HPLC
• Pore volume: 0.9 ml/g
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Eluent in column: Acetonitrile - Water
• Pore size: 110 Å
• USP listing: Not available or proprietary
• Particle size: 1.8 µm
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column type: UHPLC column
• Column volume: 0.7 ml
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Particle type: Fully porous particles (FPP)
• L x Ø internal (column): 100 x 3 mm
• Endcapped: no
• Phase: HILIC
• Temperature stability: 60 ° C
• Column shape: Analytical separation column
• pH stability: 2,0-8,5
• Specific surface according to BET: 340 m²/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Pressure stability: 900 bar