Optimized for controlled laboratory processes, this HPLC column from the Nucleodur® Hilic series features fully porous particles (FPP) with a particle size of 3 μm and a length of 100 mm, ensuring efficient separation of highly polar analytes such as amino acids, peptides, and water-soluble vitamins. With a pore size of 110 Å and a specific surface area of 340 m²/g, it offers excellent capacity and selectivity. The column's unique zwitterionic surface chemistry, based on high-purity NUCLEODUR® silica gel, enables hydrophilic interaction chromatography (HILIC), making it ideal for separating polar and ionic compounds under reversed-phase conditions. This column is suitable for temperatures up to 60°C and pressures up to 60

• Category: Separation columns for HPLC
• Pore volume: 0.9 ml/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Particle size: 3 µm
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• L x Ø internal (column): 100 x 4.6 mm
• Pore size: 110 Å
• Particle type: Fully porous particles (FPP)
• Column volume: 1.65 ml
• Column type: HPLC column
• Eluent in column: Acetonitrile - Water
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Phase: HILIC
• Endcapped: no
• USP listing: Not available or proprietary
• pH stability: 2,0-8,5
• Temperature stability: 60 ° C
• Column shape: Analytical separation column
• Pressure stability: 600 bar
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Specific surface according to BET: 340 m²/g